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| Ferreira,Catia S. M.; Missailidis,Sotiris. |
| Aptamers, short, single stranded oligonucleotide entities, have been developed in the past 15 years against a plethora of targets and for a variety of applications. These range from inhibition of receptors and enzymes to the identification of small molecules in sensor applications, and from the development of targeted therapeutic to the design of novel diagnostic and imaging agents. Furthermore, aptamers have been designed for targets that cover a wide range of diseases, from HIV to tropical diseases, cancer and inflammation. Their easy development and flexibility of use and manipulation, offers further potential. In this paper we review their selection and consider some of the recent applications of aptamers in the design of radiopharmaceuticals for the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aptamers; Radiopharmaceuticals; SELEX; Radiolabeling; Medical imaging. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600008 |
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| Rastogi,Meenal; Gupta,Sameer; Sachan,Manisha. |
| ABSTRACT Ovarian carcinoma accounts for highest mortality of all gynecologic malignancies as the disease is asymptomatic until late stages. Biomarkers such as CA-125 and HE4 are being currently used for diagnosis of ovarian cancer, but they show contradicting diagnostic accuracy. Therefore other biomarkers have been investigated for early detection of this disease, but no success has been obtained and no test has yet been recommended for screening a general population. In this instance, aptamers can be effectively used to identify tumor-specific antigens for early diagnosis and targeted therapy of ovarian cancer. This article provides an overview of the biomarkers/panels being explored as well as the potential of aptamers to improve current long-term... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovarian cancer; Aptamers; DNA methylation; CA-125; HE4. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100346 |
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| Gallo,M.; Montserrat,J.M.; Iribarren,A.M.. |
| Oligonucleotides have a wide range of applications in fields such as biotechnology, molecular biology, diagnosis and therapy. However, the spectrum of uses can be broadened by introducing chemical modifications into their structures. The most prolific field in the search for new oligonucleotide analogs is the antisense strategy, where chemical modifications confer appropriate characteristics such as hybridization, resistance to nucleases, cellular uptake, selectivity and, basically, good pharmacokinetic and pharmacodynamic properties. Combinatorial technology is another research area where oligonucleotides and their analogs are extensively employed. Aptamers, new catalytic ribozymes and deoxyribozymes are RNA or DNA molecules individualized from a randomly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Modified nucleotides; Antisense; Ribozymes; Oligonucleotide libraries; Aptamers; SELEX method. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200001 |
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| Coussot, Gaelle; Le Postollec, Aurelie; Incerti, Sebastien; Baque, Mickael; Faye, Clement; Vandenabeele Trambouze, Odile; Cottin, Herve; Ravelet, Corinne; Peyrin, Eric; Fiore, Emmanuelle; Vigier, Flavie; Caron, Jerome; Chaput, Didier; Przybyla, Bartos; Berger, Thomas; Dobrijevic, Michel. |
| Some microarray-based instruments that use bioaffinity receptors such as antibodies or aptamers are under development to detect signatures of past or present life on planetary bodies. Studying the resistance of such instruments against space constraints and cosmic rays in particular is a prerequisite. We used several ground-based facilities to study the resistance of aptamers to various types of particles (protons, electrons, neutrons, and carbon ions) at different energies and fluences. We also tested the resistance of aptamers during the EXPOSE-R2 mission outside the International Space Station (ISS). The accumulated dose measured after the 588 days of this mission (220 mGy) corresponds to the accumulated dose that can be expected during a mission to... |
| Tipo: Text |
Palavras-chave: Astrobiology; Cosmic rays; Biochip; Aptamers. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00601/71306/69723.pdf |
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| Ulrich,H.; Alves,M.J.M.; Colli,W.. |
| Recent research has shown that receptor-ligand interactions between surfaces of communicating cells are necessary prerequisites for cell proliferation, cell differentiation and immune defense. Cell-adhesion events have also been proposed for pathological conditions such as cancer growth, metastasis, and host-cell invasion by parasites such as Trypanosoma cruzi. RNA and DNA aptamers (aptus = Latin, fit) that have been selected from combinatorial nucleic acid libraries are capable of binding to cell-adhesion receptors leading to a halt in cellular processes induced by outside signals as a consequence of blockage of receptor-ligand interactions. We outline here a novel approach using RNA aptamers that bind to T. cruzi receptors and interrupt host-cell... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: SELEX; Aptamers; Cell adhesion; Cell invasion; Trypanosoma cruzi. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300002 |
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